Fascination About attune flow cytometer
Fascination About attune flow cytometer
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Data-enriched characterization of cell populations. Morphological details from pictures can add towards the richness of flow cytometry info. One example is, the determine reveals an in any other case regular apoptosis assay making use of Annexin V and PI, adding cell imaging to characterize cells in Every population to expose morphologically distinct attributes. These insights could not have been acquired from multiplex staining alone.
As samples are acquired around the Attune CytPix Flow Cytometer, the substantial-pace brightfield digicam captures and retailers photos of detected gatherings at a fee as much as 6,000 pictures for each 2nd, based on the flow price and graphic dimensions. For larger flexibility, Attune Cytometric Program enables you to regulate the impression capture frequency as essential and choose a specific gate for graphic capture, if required.
Blend acoustic focusing technological know-how with superior-parameter Investigation for flexible spectral unmixing and traditional workflows.
Find out new insights into your experimental knowledge by visualizing populations utilizing blended fluorescence and morphology info. Our software interface wizards permit downsampling, concatenation (merged documents) and dimensionality tools that are created into the application for an intuitive, streamlined knowledge Assessment working experience.
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Additionally, it really is illuminating to discover how many doublets slip via standard singlet gates, and great to validate the status of sudden double-positive situations in my Investigation."
Optimizing gating approach. Even strong manual singlet gating is error-inclined and stays a subjective conclusion issue in Virtually all flow cytometry assays. Imaging can be utilized to confirm and adjust gates to include only solitary cells of curiosity.
Our scientists will structure panels to suit your needs as many times you would like—gratis, no product or service buy wanted.
Early signs of this contamination had originally been dismissed as particles. Backgating to find out new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also enables you to use morphological characteristics to find out intriguing subpopulations that may not be apparent from flow cytometry details on your own.
Possibly most importantly, these events include cells of Obviously unique phenotypes which could lead to incorrect conclusions regarding double beneficial situations (especially in unusual populations).
AI-driven automated impression Evaluation with the Attune Cytometric Software interprets occasion attributes into unique morphology parameters which can be coupled with typical fluorescence and scatter parameters to expose extremely reproducible inhabitants variations and exclusive subsets.
Software Notice: Automated image analysis minimizes consumer-to-user variability in flow cytometry gating tactics
Leverage the strength of Innovative technological innovation and innovation to accelerate your discoveries. Have confidence in from the Attune Flow Cytometer relatives to provide the trustworthiness, pace, and precision that set your operate apart.
No clean/no lyse leukocyte Evaluation with imaging. Cells were get more info being obtained from 24-hour-previous blood by dilution in 1 mM EDTA ( Professional UX/UI permits users to educate AI styles for expanded programs. (A) The “Prepare Design” attribute gives annotation applications and real-time product schooling precision.
Gating on these extended parameters allows you to quickly and precisely recognize populations of curiosity and make sure gating technique with little if any handbook evaluate. The Attune software presents usage of state-of-the-artwork machine Studying Investigation equipment in an expertly made user interface, which makes it extremely easy to use.
Because the cell line had Formerly been analyzed to the Attune CytPix Flow Cytometer, the researchers went back again to the images and were in a position to doc the microbial an infection at the least five times before.
The Attune CytPix Flow Cytometer allows buyers to visualize samples all through acquisition, confirm the precision of gating methods, and support effects with trusted documentation for publication applications.
What appeared to be a homogeneous population is definitely much more various—an insight that ought to be considered when interpreting results.